CONFERENCE PROCEEDING
Synthesis of methylphosphorylated oligomannosides structurally related to lipopolisaccharide O-antigens of Klebsiella pneumoniae serotype O3 and the study of their immunologic properties
1
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2
I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia
Publication date: 2024-11-26
Public Health Toxicol 2024;4(Supplement Supplement 2):A20
KEYWORDS
ABSTRACT
Introduction:
Klebsiella pneumoniae causes community- and healthcare-associated infections in children and adults. Globally in 2019, an estimated 4.95 million deaths were associated with bacterial antimicrobial resistance (AMR), and K. pneumoniae has the leading position among such pathogens1. The vaccines are effective means to reduce AMR, and this report discusses the approaches the development of 3rd generation carbohydrate vaccines (synthetic conjugate vaccines) against K. pneumoniae serotype O3.
Methods:
The original methods of stereospecific synthesis of oligosaccharides developed in our laboratory made it possible to obtain preparative amounts of oligosaccharide ligands corresponding to immunodeterminant fragments of K. pneumoniae O3 antigens2-4. These oligosaccharides were used to produce coating reagents for ELISA (biotinylated conjugates) and immunogens (conjugates with BSA) to produce specific antibodies.
Results:
In the present work, pentasaccharide fragments of the terminal site of the O-chain of LPS of the bacterium K. pneumoniae O3 with and without a phosphate group were synthesized. The obtained compounds allowed us to study the immunological properties of the Klebsiella target antigen, in particular the recognition and formation of specific immunoglobulins, which emphasized the pronounced immunogenic properties of the mannoside fragments containing a methylphosphate group.
Conclusions:
The described compounds together with other K. pneumoniae related antigenic oligosaccharides could be potentially used as molecular probes for K. pneumoniae serological diagnostics development and strain serotyping.
Conflicts of interest:
The authors declare that they have no conflict of interest in the publication of this article. The authors have no conflicts of interest to report in this work. Abstract was not submitted elsewhere and was first published here.
Funding:
This work was supported by the Ministry of Science and Higher Education of the Russian Federation (Theme No. FFZZ-2022-0010) for new laboratories in the direction of ‘New Medicine’.
Klebsiella pneumoniae causes community- and healthcare-associated infections in children and adults. Globally in 2019, an estimated 4.95 million deaths were associated with bacterial antimicrobial resistance (AMR), and K. pneumoniae has the leading position among such pathogens1. The vaccines are effective means to reduce AMR, and this report discusses the approaches the development of 3rd generation carbohydrate vaccines (synthetic conjugate vaccines) against K. pneumoniae serotype O3.
Methods:
The original methods of stereospecific synthesis of oligosaccharides developed in our laboratory made it possible to obtain preparative amounts of oligosaccharide ligands corresponding to immunodeterminant fragments of K. pneumoniae O3 antigens2-4. These oligosaccharides were used to produce coating reagents for ELISA (biotinylated conjugates) and immunogens (conjugates with BSA) to produce specific antibodies.
Results:
In the present work, pentasaccharide fragments of the terminal site of the O-chain of LPS of the bacterium K. pneumoniae O3 with and without a phosphate group were synthesized. The obtained compounds allowed us to study the immunological properties of the Klebsiella target antigen, in particular the recognition and formation of specific immunoglobulins, which emphasized the pronounced immunogenic properties of the mannoside fragments containing a methylphosphate group.
Conclusions:
The described compounds together with other K. pneumoniae related antigenic oligosaccharides could be potentially used as molecular probes for K. pneumoniae serological diagnostics development and strain serotyping.
Conflicts of interest:
The authors declare that they have no conflict of interest in the publication of this article. The authors have no conflicts of interest to report in this work. Abstract was not submitted elsewhere and was first published here.
Funding:
This work was supported by the Ministry of Science and Higher Education of the Russian Federation (Theme No. FFZZ-2022-0010) for new laboratories in the direction of ‘New Medicine’.
REFERENCES (4)
| ISSN: | 2732-8929 |
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