Marrying inorganics and biologics: Opportunities and challenges
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Ιnstitute of Pharmacy and Food Chemistry, Julius-Maximilians-Universität Würzburg, Würzburg, Germany
Multi-Scale Robotics Lab, ETH Zürich, Zürich, Switzerland
Publication date: 2021-09-27
Public Health Toxicol 2021;1(Supplement Supplement 1):A51
Stroke is the second leading cause of death worldwide. The gold standard for the emergency treatment of ischemic stroke is recombinant tissue plasminogen activator (tPA). However, its use is associated with serious side effects e.g. internal bleeding, which drives the effort for the development of targeted tPA delivery platforms. Some of the most promising active delivery strategies encompass the use of microdevices based on inorganic materials. Despite the promise of achieving synergistic effects, the consequences of the interactions between complex biological molecules and inorganic materials need careful investigation. Among them is the unspecific adsorption of tPA to inorganic surfaces. Co-incubation of nanoparticles and tPA lead to a reduction in enzyme recovery as investigated by SDS gel electrophoresis. Interestingly application of a fluorescence activity assay showed that at least part of the adsorbed enzyme retained its catalytic activity of 58% +/-3%. These findings have two-fold implications for future development of tPA associated microdevices. On one side their interactions could be monitored and optimized by surface treatments to achieve minimal adsorption and inactivation. On the other side adsorption of the active molecule could be employed for site-specific delivery with release kinetics determined by physiochemical interactions.
The authors acknowledge support from the European Union's Horizon 2020 research and innovation programme under grant agreement No 952152, project ANGIE (MAgnetically steerable wireless Nanodevices for the tarGeted delivery of therapeutIc agents in any vascular rEgion of the body).
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