Hydrogel matrix coatings maintain a high moisture content in the wound area and provide gas exchange between the wound and the external environment. Such properties as biocompatibility, rapid exudate absorption and reduction of wound site temperature attract academic and industrial interest. These experiments were carried out on Amph-PVP with different molecular weight (10000, 25000, 120000 Da) and hyaluronic acid hydrogels (weight 0,059 ×10 6, ; 1,3 ×10 6, Da).The hydrogels cross-linked by the different methods using both chemical and physical crosslinking routes. Different crosslinking methods can be implemented for the design of a hydrogel. Amph-PVP hydrogel was synthesize by Gamma and electron beam polymerization. However, chemical cross-linking is a highly versatile method to improve the mechanical property of the hydrogels based on polysaccharides. So hydrogel with hyaluronic acid cross-linked by using BDDE or Polyethylene glycols. In our investigation, we studied the structural and mechanical properties of hydrogels and used Gel permeation chromatography (GPC) to determine the degree of crosslinking. The analysis was performed on a Stayer chromatograph with a Waters 2414 refractometric detector. Calibration was performed using samples of polyethylene glycol and hyaluronic acid. The obtained hydrogels are characteristic of thixotropic systems, which indicates the formation of gel structures. The initial contains several fractions with different molecular weights, while PVP were characterized by the presence of a single fraction with a wide distribution. As a result of irradiation, the molecular weight of PVP increases, which indicates its crosslinking. Through this work, we present help in the future to obtain medical materials with the desired properties. For further investigation as medical system for biomedical applications.

Acknowledgement:
The study was funded by State Assignment of the Ministry of Science and Higher Education of the Russian Federation.

Conflicts of Interest:
The authors declare that they have no conflict of interest in the publication of this article. The authors have no conflicts of interest to report in this work. Abstract was not submitted elsewhere and published here firstly.