Fluorescence polarization immunoassay for microcystin-lr
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Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow, Russia
A. N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Japan
Faculty of Engineering, Hokkaido University, Sapporo, Japan
Publication date: 2022-05-27
Public Health Toxicol 2022;2(Supplement Supplement 2):A5
Phycotoxins produced by several algae, microalgae, and cyanobacteria, are highly toxic contaminants of environmental water and sea foods. They cause necessity of efficient techniques for their simple and rapid detection. In the study, fluorescence polarization immunoassay (FPIA) was developed for microcystin-LR (MC-LR), one of the most widespread phycotoxins. Several fluorescent derivatives of MC-LR were synthesized and compared for this purpose. The reached detection limit for MC-LR was 7.5 ng/mL, and the working range of determined concentrations was 50–500 ng/mL. The developed FPIA provides rapid (10–30 min, including sample preparation) and sensitive testing. The developed portable detectors of fluorescence polarization allow testing environmental objects directly at the sampling points and making decisions about their contamination and safety rapidly.
This study was supported by the Russian Science Foundation (Project: 20-43-07001, reactants obtaining and assay optimization) and the Strategic International Collaborative Research project promoted by the Ministry of Agriculture, Forestry and Fisheries, Tokyo, Japan (development of fluorescence polarization detectors).
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