CONFERENCE PROCEEDING
Determination of macrolid antibiotics in water by fluorescence polarization method
1
Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia
2
Belozersky Research Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia
Publication date: 2026-07-30
Public Health Toxicol 2026;6(Supplement 1):A2
KEYWORDS
ABSTRACT
Introduction:
The use of antibiotics in agriculture leads to their entry into food and the environment. The problem of water pollution attracts much attention, since antibiotics are not completely removed from wastewater at treatment facilities and end up in drinking water. In veterinary medicine, macrolide antibiotics are often used - tylosin (TYL) and erythromycin (ERY). Therefore, the determination of these antibiotics in the aquatic environment becomes an urgent problem. Immune methods of analysis are widely used in laboratories in various fields of medicine - enzyme immunoassay, FPIA and immunochromatographic analysis. The FPIA method allows for high specificity and sensitivity in determining low-molecular analytes in a homogeneous medium without separation, has the ability to automate the analysis system, high accuracy, label stability, as well as speed and ease of analysis.
Methods:
The conjugates of TYL and ERY derivatives with BODIPY-FL-C5-OSu were obtained, purified and characterized. The FPIA methods were developed for determination of TYL and ERY. The FP signal was measured by portable fluorimeter Sentry-200.
Results:
In this work, fluorescently labeled tracers were obtained for determining tylosin and erythromycin with the classic fluorescein label and with the new BODIPY label. Pairs of immunoreagents (tracers and antibodies) for determining these antibiotics were selected and characterized. The conditions for conducting FPIA were optimized, calibration curves were obtained and the analytical characteristics of the method were determined: detection limit, range of detectable concentrations, sensitivity and cross-reactivity. Using the developed FPIA methods for determining antibiotics most commonly used in veterinary medicine, water samples taken from reservoirs in Moscow and the Moscow region were tested.
Conclusion:
Thus, in this work, methods for determining tylosin and erythromycin by the FPIA method with a detection limit of 5 and 1.6 ng/ml, respectively, were developed, the advantages of using the new fluorescent label BODIPY were demonstrated, the accuracy of the method was verified by the added-found test, and real water samples were tested.
The use of antibiotics in agriculture leads to their entry into food and the environment. The problem of water pollution attracts much attention, since antibiotics are not completely removed from wastewater at treatment facilities and end up in drinking water. In veterinary medicine, macrolide antibiotics are often used - tylosin (TYL) and erythromycin (ERY). Therefore, the determination of these antibiotics in the aquatic environment becomes an urgent problem. Immune methods of analysis are widely used in laboratories in various fields of medicine - enzyme immunoassay, FPIA and immunochromatographic analysis. The FPIA method allows for high specificity and sensitivity in determining low-molecular analytes in a homogeneous medium without separation, has the ability to automate the analysis system, high accuracy, label stability, as well as speed and ease of analysis.
Methods:
The conjugates of TYL and ERY derivatives with BODIPY-FL-C5-OSu were obtained, purified and characterized. The FPIA methods were developed for determination of TYL and ERY. The FP signal was measured by portable fluorimeter Sentry-200.
Results:
In this work, fluorescently labeled tracers were obtained for determining tylosin and erythromycin with the classic fluorescein label and with the new BODIPY label. Pairs of immunoreagents (tracers and antibodies) for determining these antibiotics were selected and characterized. The conditions for conducting FPIA were optimized, calibration curves were obtained and the analytical characteristics of the method were determined: detection limit, range of detectable concentrations, sensitivity and cross-reactivity. Using the developed FPIA methods for determining antibiotics most commonly used in veterinary medicine, water samples taken from reservoirs in Moscow and the Moscow region were tested.
Conclusion:
Thus, in this work, methods for determining tylosin and erythromycin by the FPIA method with a detection limit of 5 and 1.6 ng/ml, respectively, were developed, the advantages of using the new fluorescent label BODIPY were demonstrated, the accuracy of the method was verified by the added-found test, and real water samples were tested.
CONFLICTS OF INTEREST
The authors declare that they have no conflict of interest in the publication of this article. The authors have no conflicts of interest to report in this work.
The abstract was not submitted elsewhere and published here firstly.
FUNDING
The work was supported by the state task of Lomonosov Moscow State University 123032300028-0. The work was carried out with the financial support of the Lomonosov Moscow State University, project No. 1210415000398.
| eISSN: | 2732-8929 |
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