CONFERENCE PROCEEDING
Determination of dibutyl phthalate in natural waters and plastic extracts by fluorescence polarization method
1
Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia
2
National Scientific Center for “Toxicological and Biological Safety of Medical Devices" Limited, Moscow, Russia
3
Northern Water Problems Institute of Karelian Research Centre of the Russian Academy of Sciences, Petrozavodsk, Russia
Publication date: 2026-07-30
Public Health Toxicol 2026;6(Supplement 1):A7
KEYWORDS
ABSTRACT
Introduction:
Dibutyl phthalate (DBP) is widely used as a plasticizer in the production of polymeric materials to impart flexibility, strength, and extensibility. Due to its negative impact on human health, DBP content should be monitored in food and environmental objects. The aim of the work was to develop a method for polarization fluorescence immunoassay (FPIA) and to test the method using the example of analyzing natural waters of Lake Onega and its tributaries, as well as extracts from plastic.
Methods:
New immunoreagents for FPIA were synthesized - a conjugate of a succinic derivative of DBP with a fluorescent label 5-aminomethylfluorescein (AMF) and dichlorotriazinylaminofluorescein (DTAF). The DBP-AMP and DBP-DTAF was purified by thin-layer chromatography using methanol: chloroform in a ratio of 1:4 (v/v) as an eluent. The FPIA methods were developed for determination of DBP. The FP signal was measured by portable fluorimeter Sentry-200.
Results:
We have developed an FPIA method that allows rapid and specific quantitative analysis of DBP in water. In this work, we used immunoreagents to determine DBP: monoclonal antibodies against DBP and a conjugate of the succinic derivative of DBP with AMF and DTAF. A calibration dependence of DBP detection in water was obtained. The volume of water sample in the analysis was 0.1 ml, while the sample dilution factor was 10 times, which allows reducing sample preparation by only filtering water from macroparticles. The detection limit of this method was 20.0 ng / ml, which is significantly lower than the maximum permissible concentration of DBP in water established in Russia. The obtained calibration dependence does not require repetition for each sample analysis, but can be used for a selected pair of immunoreagents.
Conclusion:
The advantages of this homogeneous FPIA method are the almost complete absence of sample preparation, simple analysis, which comes down to mixing reagents and measuring the signal, and a low detection limit of 20 ng/ml. The FPIA method can be used as a cheaper and more express alternative to instrumental methods for analyzing natural waters.
Dibutyl phthalate (DBP) is widely used as a plasticizer in the production of polymeric materials to impart flexibility, strength, and extensibility. Due to its negative impact on human health, DBP content should be monitored in food and environmental objects. The aim of the work was to develop a method for polarization fluorescence immunoassay (FPIA) and to test the method using the example of analyzing natural waters of Lake Onega and its tributaries, as well as extracts from plastic.
Methods:
New immunoreagents for FPIA were synthesized - a conjugate of a succinic derivative of DBP with a fluorescent label 5-aminomethylfluorescein (AMF) and dichlorotriazinylaminofluorescein (DTAF). The DBP-AMP and DBP-DTAF was purified by thin-layer chromatography using methanol: chloroform in a ratio of 1:4 (v/v) as an eluent. The FPIA methods were developed for determination of DBP. The FP signal was measured by portable fluorimeter Sentry-200.
Results:
We have developed an FPIA method that allows rapid and specific quantitative analysis of DBP in water. In this work, we used immunoreagents to determine DBP: monoclonal antibodies against DBP and a conjugate of the succinic derivative of DBP with AMF and DTAF. A calibration dependence of DBP detection in water was obtained. The volume of water sample in the analysis was 0.1 ml, while the sample dilution factor was 10 times, which allows reducing sample preparation by only filtering water from macroparticles. The detection limit of this method was 20.0 ng / ml, which is significantly lower than the maximum permissible concentration of DBP in water established in Russia. The obtained calibration dependence does not require repetition for each sample analysis, but can be used for a selected pair of immunoreagents.
Conclusion:
The advantages of this homogeneous FPIA method are the almost complete absence of sample preparation, simple analysis, which comes down to mixing reagents and measuring the signal, and a low detection limit of 20 ng/ml. The FPIA method can be used as a cheaper and more express alternative to instrumental methods for analyzing natural waters.
CONFLICTS OF INTEREST
The authors declare that they have no conflicts of interest in the publication of this article. The authors have no conflicts of interest to report in this work. The abstract was not submitted elsewhere and published here firstly.
FUNDING
The work was carried out with the financial support of the Lomonosov Moscow State University, project No. 1210415000398.
Water sampling in the waters of Lake Onega and its tributaries was carried out using federal budget funds to fulfill the state assignment of the Karelian Research Center of the Russian Academy of Sciences (121021700118-0).
| eISSN: | 2732-8929 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
