Synthesis of complex dressings containing various therapeutic agents based on modified cellulose and their properties studying
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Mendeleev University of Chemical Technology of Russia, Moscow, Russia
Publication date: 2021-09-27
Public Health Toxicol 2021;1(Supplement 1):A45
In wound therapy, it is customary to adhere to the T.I.M.E. rule. That stands for tissue debridement, inflammation/infection, moisture imbalance, epithelial edge advancement. In this regard, it is necessary to create a dressing with a complex effect.
Biopolymers, especially polysaccharides, are known to be used in the treatment of purulent wounds due to their biocompatibility, biodegradability, and low toxicity. To that end, dialdehyde cellulose (DAC) was chosen as a matrix for the immobilization of subsequent therapeutic agents in our research work.
The most important property of wound healing dressings is their ability to biodegrade without the formation of toxic decay products. Consequently, experiments to study the kinetics of dressings' destruction were carried out.
Importantly, the process of transition of low-molecular-weight products formed by periodate oxidation of cellulose into a solution was mentioned by many authors. In particular, there is an opinion that during the high-temperature destruction of DAC, formaldehyde is formed. The data obtained by HPLC methods on the composition of the products of hydrolytic degradation are difficult to identify and do not give an unambiguous answer as to what exactly are the products of hydrolytic degradation of the support.
The analysis data obtained utilizing capillary electrophoresis by the method of additions showed that the peak of the formaldehyde yield is outside the region of retention of degradation products. During titration of the studied enzyme preparations with the destruction products of the studied polysaccharide carriers, monomer units and formaldehyde, it was shown that low molecular weight aldehydes (for example, formaldehyde) reduce the activity of proteases in concentration dependence by binding, apparently, to the amino groups of the active center of the enzyme. It was shown that the products of hydrolytic destruction of various test carriers do not affect the proteolytic activity of the studied enzymes, regardless of the exposure time, similar to glucose solutions, or have an activating effect.
The results of the experimental toxicological studies of the samples of the used cellulose materials allow us to conclude that the studied samples do not have toxic, allergic effects, as well as mutagenic activity.