Investigation of the indicative properties of Daucus carota extract in a complex system based on polysaccharides
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D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia
Publication date: 2021-09-27
Public Health Toxicol 2021;1(Supplement 1):A41
Currently, research is being actively conducted to find natural dyes and indicators, since they are safer to use for humans and renewable. Anthocyanins, curcumin, chlorophyll, carotenoids, brasiline, and tannins are often used as natural pH indicators Natural indicators are widely used in films, xerogels and coatings based on polysaccharides of various origins for pH control in food and medical products. Polysaccharides are able to absorb vapors or moisture, and the indicator, immobilized in the polysaccharide matrix, reacts to changes in pH in situ, acquiring the appropriate color. The use of acid-base indicators in the diagnosis of the condition of wounds can provide information about the healing stage. Using the properties of polysaccharides to absorb moisture, a formula of a polyethylene oxide-based ointment containing a mixture of anionic polysaccharides of sodium alginate and kappa-carrageenan (1:3) was proposed, in which a dry extract of Daucus carota (1%) was introduced. The behavior of anthocyanins from Daucus carota extract in the matrix of hydrophilic ointment based on marine polysaccharides was studied in model media with different pH (pH 5,0, 6,0, 7,0, 8,5, 10,0). Anthocyanins are sensitive to changes in pH: in an acidic environment they show various shades of red, in a neutral environment shades of purple, in an alkaline environment from blue to yellow-green. In the experiments, the time of contact with the model solution and the ratio of the model solution and the indicator ointment were changed (10:1 and 20:1). The color change occurred quite quickly, but at a ratio of the model solution and the ointment of 10:1 due to the limited amount of the model liquid, there was an incomplete swelling of the sorbing complex in the ointment, which in some cases contributed to an uneven color distribution. The indicator ability of the ointment when applied to wounds will depend on the amount of exudate released and the applied ointment. The condition of the wound can be determined both by the express method (after 10 minutes) when applying the ointment to the wound with a thin layer, and when changing the dressing after 6, 12 or 24 hours.